Chapter 9 · Part 2

Biotechnology: Principles and Processes

Recombinant DNA, restriction enzymes, PCR & cloning vectors.

Concepts

Formulae

6 min

Read time

Advanced

Learning objectives

1Explain principles of Genetic Engineering
2Explain tools — Restriction Enzymes
3Explain tools — Cloning Vectors and Hosts
4Explain steps in Making Recombinant DNA
5Explain amplifying DNA — PCR
6Explain bioreactors and Downstream Processing

Your learning path

Introduction

Principles of Genetic Engineering

Tools — Restriction Enzymes

Tools — Cloning Vectors and Hosts

Steps in Making Recombinant DNA

Amplifying DNA — PCR

Bioreactors and Downstream Processing

Summary

Quiz

Revision

Follow the full path

Key terms

BiotechnologyRestriction enzymesDNAPCRplasmidCuttingLigationIsolationannealingextensionDNA ligaseHost cellsbioreactorpalindromicsticky endsBioreactors

Connected chapters

Molecular Basis of Inheritanceshares: DNA, DNA ligase Biotechnology and Its Applicationsshares: PCR

6Concepts

0Formulae

6Minutes

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